Job Description

Title: Scientist III

Location: Ridgefield, CT 06877

Duration:12 Months

Note:

• Must have 2 years of Crispr experience.
• Molecular biology, qPCR, western blot. Proficiency in cell culture. Primary cell lines.
• Design guide RNA for a target, order a guide RNA, do the cell culture, grow cells, they do the electroporation, coordinate CRO work, submit samples on behalf of other Tas.
• They teach them single-cell-seq
• Every day will be cell culture work; QPCR, western blot
• If you have candidates who are willing to relocate at their own expense, we are willing to consider them as well.

Job Duties:

• Design, develop, and execute functional genomics assays, including CRISPR-based editing (e.g., RNP delivery, Perturb-seq), single-cell sequencing, spatial transcriptomics, and CITE-seq.
• Provide technical guidance and training to research scientists on FGSL technologies and techniques, including assay execution, troubleshooting, and resolution of technical issues;
• Documents and maintains detailed laboratory records in accordance with policies;
• Operates and maintains lab equipment in collaboration with other lab members;
• Assists acquisition of necessary supplies and equipment;
• Defines and performs appropriate QC measures;
• Follows relevant scientific literature to ensure use of optimal methods and understand emerging practices across the field;
• Demonstrates the ability to interpret the outcome of experiments, propose appropriate follow-up, and may propose new avenues of investigation;
• Communicates own work effectively orally and in writing; contributes to writing protocols, procedures, and technical reports
• Automate processing and results reporting and delivery;
• Reports and treats data with a high level of integrity and ethics.

Skills:

• CRISPR assays - Must have personally designed guides, performed transfections/infections, selected edited cells, and validated knockouts/knockins
• Next-generation sequencing (NGS) - Hands-on library preparation experience (not just data analysis)
• Single cell technologies - Personal experience with sample prep, library generation, and quality control
• Standard molecular biology techniques - qPCR, Western blot, cloning, gel electrophoresis
• Cell culture - Proficient in maintaining multiple cell lines, transfections, and sterile technique
• Experience in automation is a plus;
• Able to perform variety of FGS experimental techniques at the same time;
• Demonstrated experience working in a multi-disciplinary and collaborative environment;
• Ability to troubleshoot FGS experiments both individually and as part of a team.
• Excellent oral and written skills with the ability to communicate in an open, transparent, timely and consistent manner.

Education:

• Master’s degree from an accredited institution with 4+ years of experience in molecular, cell biology, or related scientific discipline

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